A model approach to distinguish diploid and triploid fish by means of computer-assisted image analysis

Abstract

A model approach to distinguish diploid and triploid fish rapidly upon computer-assisted image analysis of erythrocyte nuclear dimensions in blood smear samples was exemplified on measurements of 13 diploid and 13 artificially-induced triploid wels (Silurus glanis). Analysis of variance test confirmed that nuclear area, perimeter, nuclear major axis and average nuclear size were the decisive dimensions for such approach (P < 0.0001). The respective values (mean ± SD) for diploids and triploids were registered as follows: nuclear area 11.09 ± 1.26 μm2 and 17.34 ± 2.09 μm2; nuclear perimeter 12.67 ± 0.80 μm and 16.24 ± 1.07 μm; nuclear major axis 4.62 ± 0.35 μm and 6.17 ± 0.47 μm and average nuclear size 4.01 ± 0.22 μm and 5.12 ± 0.31 μm. This approach was found a rapid and precise alternative to other currently used methods of ploidy level identification in fish.