Acute and chronic cyclooxygenase blockade in portal-hypertensive rats: Influence on nitric oxide biosynthesis

Abstract

Background and Aims: Prostacyclin and nitric oxide have been involved in the hyperkinetic syndrome in portal hypertension. The aim of this study was to investigate the relative contribution and possible interaction between prostacyclin and NO in this circulatory abnormality. Methods: Portal vein- ligated and sham-operated rats received indomethacin and vehicle either on a short-term (5 mg/kg subcutaneously) or long-term basis (5 mg · kg-1 · day-1, continuous 7-day infusion with an osmotic minipump). Measurements of arterial pressure and superior mesenteric arterial flow (mL · min-1 · kg-1, ultrasonic flow probe) were then performed before and after N(G)- nitro-L-arginine methyl ester (L-NAME) injection (13 mg/kg intravenously). Results: Short-term or long-term indomethacin treatment had no effect in sham-operated rats but significantly decreased mesenteric arterial flow in portal-hypertensive rats. Mesenteric flow remained higher after long-term than after short-term indomethacin treatment (54.5 ± 2 vs. 46.1 ± 2; P = 0.01). This blunted response to long-term indomethacin treatment was associated with an enhanced response to L-NAME, shown by greater increments in arterial pressure (29% ± 3%) and mesenteric arterial resistance (209% ± 22%) in indomethacin-treated rats than in rats receiving vehicle (19% ± 2% and 130% ± 20%; P < 0.05). Conclusions: Both prostacyclin and NO contributed to splanchnic hyperemia in portal-hypertensive rats. There was an enhanced release of NO after long-term prostacyclin inhibition, suggesting that both vasodilatory systems interact, promoting splanchnic hyperemia in portal hypertension.