CMA banding patterns of chromosome of mid- and late- maturing citrus and acid citrus grown in Japan

Abstract

Fluorochrome staining with chromomycin A 3 (CMA) was used to characterize and compare the CMA banding patterns of chromosomes in eight species of citrus that are cultivated in Japan. Chromosomes were classified into five types based on the number and position of CMA positive bands; A: two terminal and one proximal band, B: one terminal and one proximal band, C: two terminal bands, D: one terminal band, E: no band. The CMA banding patterns were 1A+1C+8D+8E in Hassaku, 2A+2C+5D+9E in Hyuganatsu, 1A+2C+7D+8E in Natsudaidai 'Kawano natsudadai', 1A+1B+1C+8D+7E in Iyo 'Miyauchi iyokan', 1A+1B+1C+8D+7E in Tankan 'Tarumizu 1 gou', 3B+2C+5D+8E in Kabosu, 1B+2C+9D+6E in Sudachi and 2B+1C+11D+4E in Yuzu 'Yamane'. A similar CMA banding pattern was observed in related species examined in this study.