Phytochemical profiling of methanolic extract of Periploca aphylla Decne. using GC-MS analysis

Abstract

Introduction: Periploca aphylla Decne. (Asclepiadoideae) locally named as “Bata”, is a medicinally important plant. The milky juice of P. aphylla is externally applied to tumors and swellings, for cerebral fever treatment and as stomachic. The flowers are used as vegetables, emetic purposes, expectorant, laxative, diuretic and for wart removal. Objectives: In this study, crude methanolic extract of P. aphylla (whole plant) was investigated for its phytochemical composition using GC-MS analysis. Methodology: THERMO GC-TRACE ULTRA VER: 5.0, THERMO MS DSQ II and Gas chromatograph interfaced to a Mass spectrometer (GC-MS) equipped with a ZB 5-MS CAPILLARY STANDARD NON-POLAR COLUMN (30 m x 0.25 mm ID x 0.25 μm FILM) was used to carry out analysis of extract. The interpretation of mass spectrums was performed utilizing National Institute of Standard and Technology (NIST) database. Results: Thirty compounds were recognized in P. aphylla methanol extract during GC-MS analysis in which hexadecanoic acid, methyl ester (13.65%) was a major component followed by 1,1,6-trimethyl-3-methylene-2-(3,6,9,13 tetramethyl-6-ethenye-10,14-dimethylene-pentadec-4-enyl)cyclohexane (9.41%), 9-Octadecenal, (Z)-(CAS) (8.00%), Pyrrolidine, 1-(6-phenyl-1-cyclohexen-1-yl)-(CAS) (7.50%) and 5 (Dimethylamino)-3-ethoxy-5-isopropyl-1-(4'-trifluoromethylphenyl)-2-[2"-(4-"'trifluoromethyl phenyl) ethynyl]-1,3-cyclopentadiene (5.28%). Conclusion: Periploca aphylla is a rich source of chemical compounds having therapeutic potentials which validates its usage for the treatment of different diseases.