Anticancer Activity Test Of 70% Alcohol Extract of Sembung Leaves (Blumea Balsamifera) Against Cervical Cancer Cells In-Vitro

Abstract

Cervical cancer is one of the most common cancers among Indonesian women, second only to breast cancer. Several anticancer medications cause minor to severe adverse effects such as fever, nausea, vomiting, hair loss, and other diseases. Because of the negative side effects suffered by patients, numerous researchs have been done to investigate herbal medicines as alternative cancer therapies with less side effects and more efficacy. The purpose of this study is to assess the efficacy of a 70% alcohol extract of Blumea Balsamifera (Sembung leaf) against cervical cancer cells. This study used a true experimental design with a quantitative methodology. The study used a 70% alcohol extract of Blumea Balsamifera leaves with concentrations of 15.625, 31.75, 62.5, 125, and 250 mg/ml, as well as HeLa cell cultures. Absorbance values were determined using the MTT assay and an ELISA Reader. Significant differences were discovered at concentrations of 125 and 250 mg/ml, while no significant differences were seen at concentrations of 15.625 and 31.25 mg/ml (p > 0.05). The study found that a concentration of 21.62 μg/ml of the extract reduces cell viability by up to 50%.