Development of tetranucleotide microsatellite markers in Falcataria moluccana

Abstract

Falcataria moluccana (Miq.) Barneby & J. W. Grimes is an important forest resource in tropical Asia Ten polymorphic tetranucleotide microsatellite markers were isolated using a ligation-mediated suppression PCR technique. The polymorphism of these markers was investigated in 33 individuals from natural populations. The number of alleles ranged from nine to 16 with an average of 12.4 alleles per marker, and the expected heterozygosities of the 10 markers ranged from 0.752 to 0.924. These polymorphic markers will be powerful tools for use in population genetics, conservation genetics, and molecular ecology studies to evaluate and conserve genetic resources and biodiversity in F. moluccana.