Confocal Microscopy System Performance: Field Illumination

Abstract

The confocal laser-scanning microscope (CLSM) has enormous potential in many biological fields. The reliability of the CLSM to obtain specific measurements and quantify fluorescence data is dependent on using a correctly aligned machine that contains a stable laser power. For many applications it is useful to know the CLSM system's performance prior to acquiring data images so the necessary resolution, sensitivity and precision can be obtained. Applications involving deconvolution, FRET and quantification necessitate that the confocal microscope is correctly configured and operating at the highest performance levels.