Genome sequence resources for the wheat stripe rust pathogen (puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (puccinia striiformis f. sp. hordei)

Abstract

Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat andP. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. triticigenomes are available, but no P. striiformis f. sp. hordei genome is available. More genomesof P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genomeevolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp.tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illuminatechnologies and RNA sequencing. Their genomic sequences were assembled to contigswith high continuity and showed significant structural differences. The circular mitochondriagenomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes foravirulence and other important traits, and studying host-pathogen interactions.