Optimization of Temperature and pH for the Protease Production By Endophytic Fungi From Mangrove Sonneratia alba

Abstract

The research about optimization of temperature and pH for the protease production from mangrove Sonneratia alba endophytic fungi in Mandeh Area, Pesisir Selatan District was conducted from March to June 2023 at Biotechnology Laboratory, Andalas University, Padang. This research aims to determine the optimum temperature and pH of endophytic fungal isolates of mangrove plants Sonneratia alba from the Mandeh area, Pesisir Selatan District in producing protease and to determine the enzyme production after optimization. The research method used in this study was experimental and descriptive.Protease activity was determined using the Takami method. The results of the study showed that the optimum temperature for isolates EUA-124 and EUA-126 in producing protease is 34°C the optimum pH for isolate EUA-124 in producing protease is 7 and the optimum pH for isolate EUA-126 in producing protease is 6. Protease production by isolates EUA-124 increased by 8.621% and protease production by isolate EUA-126 increased by 3.334%.