MODIFICATION OF THE AGRANOFF‐SUOMI METHOD FOR THE SYNTHESIS OF CDP‐DIACYLGLYCEROL

Abstract

Cytidine 5′‐diphospho‐1,2‐diacyl‐sn‐glycerol (CDP‐diacylglycerol) is an important liponucleotide intermediate in the biosynthesis of phosphatidyl‐inositol, phosphatidylserine and phosphatidylglycerophosphate. The Agranoff‐Suomi method (Agranoff, B. W. and Suomi, W. D. (1963) Biochem. Prep. 10, 47–51) for the synthesis of CDP‐diacylglycerol was modified by a procedure which eliminates the lyophilization step required for the preparation of reactants. The reaction was run in chloroform instead of pyridine using the catalyst 4‐dimethylaminopyridine. The product of the reaction was purified by silica gel column chromatography and is enzymatically active with the enzymes phosphatidylinositol synthase, phosphatidylserine synthase and phosphatidylglycerophosphate synthase. Copyright © 1980, Wiley Blackwell. All rights reserved