Prevention of biofilm colonization by gram-negative bacteria on minocycline-rifampin-impregnated catheters sequentially coated with chlorhexidine

Abstract

Resistant Gram-negative bacteria are increasing central-line-associated bloodstream infection threats. To better combat this, chlorhexidine (CHX) was added to minocycline-rifampin (M/R) catheters. The in vitro antimicrobial activity of CHX-M/R catheters against multidrug resistant, Gram-negative Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia was tested. M/R and CHX-silver sulfadiazine (CHX/ SS) catheters were used as comparators. The novel CHX-M/R catheters were significantly more effective (P<0.0001) than CHX/SS or M/R catheters in preventing biofilm colonization and showed better antimicrobial durability. Copyright © 2014, American Society for Microbiology. All Rights Reserved.