In Vitro Antioxidants Activities of Extract of Tri-Kasorn-Mas remedy and its plant ingredients

Abstract

Tri-Kasorn-Mas remedy is one of the Thai traditional medicines notified in the list of Herbal Medicinal Products A.D. 2021 of Thailand for the treatment of fatigue and maintaining body balance of patients. However, there have been no scientific reports on the antioxidant activities and antioxidant capacity. This study aimed to investigate antioxidant activities, and total phenolic and flavonoid contents of the three extracts of the Tri-Kasorn-Mas remedy and their mixture. The remedy plants and each ingredient were extracted by 95% ethanol maceration. These studies determined antioxidant activity by DPPH radical scavenging assay, ABTS radical cation decolorization assay, Ferric ion reducing antioxidant power assay (FRAB) and chemical contents by total phenolic and flavonoid content methods. The yield of Tri-Kasorn-Mas remedy was 26.68%. The results found that the Tri-Kasorn-Mas remedy showed strong antioxidant activity with an EC50 value of 9.37 ± 0.82 × 10–3 mg mL–1 by DPPH radical scavenging assay and 7.91 ± 0.01 × 10–3 mg mL–1 by ABTS radical cation decolorization assay. These extracts also were stronger activity than the positive control. In addition, J. multifida was the highest antioxidant activity than other ingredients. The results of this study can be preliminarily concluded that the Tri-Kasorn-Mas remedy has antioxidant activity and total phenolic compounds that are in high values.