COMPARISON OF ENDOPHYTIC MICROBIOME COMMUNITY AND RHIZOSPHERE IN THE DESERT PLANT SENNA ITALICA

Abstract

Senna italica is indigenous to the desert and has important ecological and economic value in Kingdom of Saudi Arabia (KSA). Plant microbial endophytes and rhizosphere contribute significantly to plant growth, development, health, and ecological function. The variety of endophytic bacteria and rhizosphere associated with S. italica is still unknown. In this work, the Illumina MiSeq sequencing of bacterial 16S rDNA was used to examine the structure of the bacterial communities associated with various tissues, including roots, leaves, and rhizosphere soils. A total of 840.242 sequences and 1142 operational taxonomic units (OTUs) were obtained. Cyanobacteria were the most abundant bacterial phylum in endophyte samples, followed by Actinobacteria; and Actinobacteria and Proteobacteria were the most abundant phyla in rhizosphere soil samples. According to predictive metagenome research, endophytic bacteria and rhizosphere serve vital functional roles in S. italica's ability to tolerate abiotic stress. This conclusion could facilitate the study of the ecological functions of endophytic bacteria and rhizosphere and their interactions with S. italica to identify novel organisms that may have a role in abiotic stress resistance in the plant. Where there is still much about the endophytes and rhizosphere microbiome that may be discovered with more study.