Circulating long noncoding RNAS H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study

35Citations
Citations of this article
31Readers
Mendeley users who have this article in their library.

Abstract

Recently, a wide range of biological and pathological roles of long noncoding RNAs (lncRNAs) have been discovered. However, the potential role of circulating lncRNAs H19 and GAS5 in type 2 diabetes mellitus (T2DM) and diabetic retinopathy (DR) is not clear. Here, we assessed the plasma levels of H19 and GAS5 lncRNAs in T2DM patients with/without DR and evaluated if H19 and GAS5 pre-treatment plasma levels are a predictor of early response to a single aflibercept dose in DR subgroup. Plasma lncRNA expression profiles of 119 T2DM patients (66 with DR and 53 without DR) and 110 healthy controls were determined by quantitative reverse transcription PCR. The association of lncRNA expression profiles with clinical features and aflibercept early response in DR patients was investigated. Relative H19 expression levels were significantly increased in T2DM group (including DR and non-DR subgroups) vs. controls, while GAS5 levels were decreased in T2DM group (p < 0.001). There was no significant difference in H19 and GAS5 expression levels between DR and non-DR subgroups. H19 and GAS5 expression profiles were not significantly correlated with clinical parameters or response to aflibercept therapy in DR subgroup. Our findings indicate that the circulating lncRNAs H19 and GAS5 may be associated with T2DM prevalence but may not have an important diagnostic/prognostic role in DR or early response to aflibercept intravitreal injection in DR patients. Large-scale transcriptomic studies are warranted to validate our results and investigate other lncRNA candidates in T2DM.

References Powered by Scopus

Analysis of relative gene expression data using real-time quantitative PCR and the 2<sup>-ΔΔC</sup>T method

150849Citations
N/AReaders
Get full text

The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments

12411Citations
N/AReaders
Get full text

Epidemiology of diabetes and diabetes-related complications

1172Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Mitochondrial dysfunction and chronic inflammation in polycystic ovary syndrome

95Citations
N/AReaders
Get full text

The Impact of lncRNAs in Diabetes Mellitus: A Systematic Review and In Silico Analyses

53Citations
N/AReaders
Get full text

Epigenetic mechanisms in type 2 diabetes retinopathy: A systematic review

42Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Fawzy, M. S., Abdelghany, A. A., Toraih, E. A., & Mohamed, A. M. (2020). Circulating long noncoding RNAS H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study. Bosnian Journal of Basic Medical Sciences, 20(3), 365–371. https://doi.org/10.17305/bjbms.2019.4533

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 7

44%

Researcher 5

31%

Lecturer / Post doc 3

19%

Professor / Associate Prof. 1

6%

Readers' Discipline

Tooltip

Medicine and Dentistry 6

40%

Biochemistry, Genetics and Molecular Bi... 6

40%

Nursing and Health Professions 2

13%

Neuroscience 1

7%

Save time finding and organizing research with Mendeley

Sign up for free