Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus

Abstract

Introduction: Herpes simplex virus (H SV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fl uid samples has allowed the detection of viral DNA with high sensitivity and specifi city. Methods: Serial dilutions of quantifi ed commercial controls of each virus were subjected to an in-house nested-PCR technique. Results: The minimum detection limits for HSV and VZV were 5 and 10 copies/μL, respectively. Conclusions: The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.