Isolation, documentation, and biochemical characterization of cellulolytic bacteria from rumen fluid of cattle

Abstract

ICLE ABSTRACT The microbiological use of cellulose is a significant aspect in achieving the highest possible material flow in the environment. This study aimed at isolation, identification, and biochemical characterization of bacteria with cellulase activity from cellulose samples. Cellulase enzyme has wide applications in various industries including food industry, agriculture, textile, detergent, pulp and paper, biofuel production, brewing and biorefinery. In the present study, cellulolytic bacterium Pseudomonas sp. was isolated from rumen fluid of cattle was collected from slaughter house. This bacterial isolate was identified by morphological, biochemical, and physiochemical characteristics. Cellulase production by the bacteria was optimized. Optimum cellulase was produced by Pseudomonas sp. which was observed under different pH, temperature, and incubation period. The highest production of cellulase enzyme by this bacterium was monitored at pH 7.0 for 48 hours under 3 days of cultivation at 40°C. The enzyme activity was observed at pH 7.0 with cellulosic filter paper for 5 days incubation and its maximum activity was noted at 48 hours. The result of study shows that the Pseudomonas sp. is a good producer of extra cellular cellulase enzyme which can be also beneficial for the degradation of cellulosic pollutants of environment.