Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria

Abstract

Antibacterial activity of ethanolic and aqueous extracts of Capsella Bursa were carried out against eight different species of bacteria, Gram-positive Staphylococcus aureus and Enterococcus fecalis and Gram-negative Escherichia coli, Proteus vulgaris, Serratia marcescens,Acinitobacter bumani, Klebsiella pneumoniae and Pseudomonas aeruginosa. It is active antibacterial only against gram-negative bacteria. The ethanolic and aqueous extract from the same plant showed different activities; the aqueous extract (hot) showed the same or greater activity than the ethanolic extract. by disc diffusion, hot aqueous extract 2000 and 3000 µg/ml inhibition zone was observed against five gram negative pathogens in almost similar pattern. Ethanolic extract was active only against Ps.aeruginosa and K. pneumoniae. The MIC and MBC values of the ethanolic and aqueous extract of Capsella Bursa was active against most bacteria. All isolates were tested by different concentration of sub-MIC of aqueous and ethanolic extracts give different activities of these concentrations to inhibit or omitting the ability of those isolates to produce virulence factors (DNase, Haemolysin production and Lipase production). Finally, the Broth dilution assay showed larger activity than the disc diffusion method.