Coffea Canephora Extract as Antibacterial Agent against Escherichia coli

Abstract

Introduction: This research is investigate antibacterial activity of Coffea canephora extract against Gram-PositiveEscherichia coli.Methods: An experimental laboratory with post-test only group design, investigating antibacterial activity ofCoffea canephora extract in concentrations 4%, 16%, 25%, 33%, 39% and 44% were observed and measured meandiameter inhibitory zone then analysed by statistical and sensitivity test. Sensitivity criteria according standard ofClinical and Laboratory Standarts Institiute (CLSI). Statistical test using Kruskal-Wallis and Mann-Whitney Test.Conclusion: In The Coffea canephora extract showed lowest concentration to inhibit Escherichia coli was 33%(Mean : 9mm) although categorized as resistant. Greatest concentration 44% (Mean: 13.75mm) was categorizedas intermediate. Statistical analysis using Kruskal-Wallis showed significant difference in all concentration andMann-Whitney showed significant mean diameter inhibitory zone in concentration 33%, 39% and 44%. In thisstudy we showed Coffea canephora extract perform antibacterial activity against Escherichia coli although in currentstudy no sensitive concentration found. Increase concentration correlated with increase mean diameter inhibitoryzone which indicated antibacterial activity.