Optimizing antioxidant substances extraction produced by Holothuria atra using ultrasonic with response surface method

Abstract

This study was to discover the effect of ultrasonic extraction based on the factors of extraction time and amplitude, to increase the yield recovery of H. atra extract and antioxidant activity (IC50). The process in optimizing ultrasonic extraction was performed using Response Surface Methodology (RSM) with Central Composite Design (CCD). H. atra extract produced by the 2 different extraction methods indicated a significant difference in yield (maceration 5.48%, ultrasonic 10.43%) and antioxidant activity (maceration 149.38 ppm, ultrasonic 84.85 ppm). The antibacterial activity against B. subtilis, E. coli, and S. aureus), also the toxicity LC50 at 457.77: 443.63 ppm were not significantly difference. It was found that ultrasonic extraction method needs shorter time with higher yield and more antioxidant activity. The sea cucumber extract that was produced from the ultrasonic method contains alkaloid, triterpenoid, and saponin, while steroid undetected, and from the maceration method contains no alkaloid and steroid.