Decoupling of Tree-Ring Cellulose δ18O and δ2H Highlighted by Their Contrasting Relationships to Climate and Tree Intrinsic Variables

Abstract

Oxygen (δ18O) and hydrogen (δ2H) stable isotope ratios are tightly coupled in precipitation and, albeit damped, in leaf water, but are often decoupled in tree-ring cellulose. The environmental and physiological conditions in which this decoupling occurs are not yet well understood. We investigated the relationships between δ18O and δ2H and tree-ring width (TRW), tree crown volume, tree age and climate in silver fir and Douglas-fir and found substantial differences between δ18O and δ2H. Overall, δ18O–δ2H correlations were weak to absent but became significantly negative under high summer vapour pressure deficit (VPD). δ18O and δ2H had positive and negative nonlinear relationships with TRW, respectively, with clear relationships at the site and tree levels for silver fir and, to a lesser extent, for Douglas-fir. Age trends for silver fir were weakly negative in δ18O but positive in δ2H. Tree crown volume and δ18O or δ2H had no significant relationships. Most strikingly, δ18O strongly depended on spring climate (precipitation and VPD), whereas δ2H depended on summer climate (temperature and VPD) for both species. Our study shows that the δ18O–δ2H decoupling in tree-ring cellulose in two temperate conifer species could be highlighted by their contrasting relationships to climate and tree intrinsic variables (TRW, age).