Production and its anti-hyperglycemic effects of γ-aminobutyric acid from the wild yeast strain Pichia silvicola UL6-1 and Sporobolomyces carnicolor 402-JB-1

Abstract

This study was done to produce γ-aminobutyric acid (GABA) from wild yeast as well as investigate its antihyperglycemic effects. Among ten GABA-producing yeast strains, Pichia silvicola UL6-1 and Sporobolomyces carnicolor 402-JB-1 produced high GABA concentration of 134.4 μg/mL and 179.2 μg/mL, respectively. P. silvicola UL6-1 showed a maximum GABA yield of 136.5 μg/mL and 200.8 μg/mL from S. carnicolor 402-JB-1 when they were cultured for 30 hr at 30°C in yeast extractpeptone- dextrose medium. The cell-free extract from P. silvicola UL6-1 and S. carnicolor 402-JB-1 showed very high antihyperglycemic a-glucosidase inhibitory activity of 72.3% and 69.9%, respectively. Additionally, their cell-free extract-containing GABA showed the anti-hyperglycemic effect in streptozotocin-induced diabetic Sprague-Dawley rats.