In-Situ Measurements of the Physiological Parameters in Lab-on-Chip Systems

Abstract

There is a growing interest in integrating biosensors into lab-on-chip systems. Sensor integration in the cell culture systems allows direct measurements of the biological and physiological parameters while the cells grow. It requires a special design of the microfluidic chip and microstructuring of the conductive materials. In this work, we present two approaches for in-situ measurements of the physiological parameters. The first approach is an integration of a commercial optical sensor for oxygen level detection. The second solution is a self-designed and self-manufactured transepithelial resistance (TEER) sensor for cell confluence measurements. Both sensors were integrated in a flexible microfluidic chip based on Polydimethylsiloxane (PDMS).