Potential polysaccharide gel from Durio zibethinus var.Raja galuh rind extract towards Klebsiella pneumoniae bacteria

Abstract

Pneumonia is commonly caused by Klebsiella pneumonia (gram negative bacteria), which is prevalent in developing countries. In 2014, from 4, 3000,143 toddlers in West Java, 48.06% of them suffered from pneumonia. Especially in Cirebon, 70.9% toddlers were diagnosed with pneumonia. The Rind of Durio zibethinus extract phenolic compounds which are believed to be able to inhibit the growth of Klebsiella pneumonia. This experimental in vitro study used 12 group samples with an experimental post-test only group design, with the following concentrations: 100%, 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, 0.39%, and 0.20%. MIC test results on the tube contain non-motile gram-negative bacteria, not encapsulated, large colonies, and very mucus and tend to collect. and total plate positive control account showed growth of bacteria number 23,66, group of 100% durian rind extract as much 25,66, group 50% that is 26,00, group 25% 22,67, group 12,5% that is 18,34, group of 6.25% is 18.00, group 3.13% is 16.34, group 1.56% is 13.67, group 0.78% is 10.67, group 0.39% 8.67, group 0.20% is 8.34 with statistical analysis p value 0,00 (significant) concentration 0,39% and 0,20% is Minimum Inhibitory Concentration (MIC) bacterium Klebsiella pneumoniae.