Evaluation of cytotoxic effect of Teucrium polium on a new glioblastoma multiforme cell line (REYF-1) using MTT and soft agar clonogenic assays

Abstract

In the present study we evaluated the in vitro cytotoxic activity of aqueous and methanolic extract of T. polium. In order to evaluate, first we established a new glioblastoma multiforme cell line, which was designated as REYF-1 cell. Cytotoxic activity of the extracts was evaluated on this cell line using both MTT assay and clonogenic cell assay. Present results show that methanolic extract of T. polium exhibits dose dependant cytotoxic effect. The IC50 values of methanolic extract were 95 and 69 μg mL-1 using MTT assay and Clonogenic assay, respectively. The IC50 for aqueous extract was 1400 μg mL-1. These findings show a cytotoxic activity of T. polium methanolic extract on glioblastorna multifortne cells in vitro. Further studies need to be done to find out the active compound(s) of this plant as a cytotoxic agent. © 2007 Asian Network for Scientific Information.