Detection and identification of a pathogenic marine Mycobacterium from the European seabass Dicentrarchus labrax using polymerase chain reaction and direct sequencing of 16S rDNA sequences.

Abstract

Mycobacteriosis has become a major concern for the commercial mariculture of the European sea bass Dicentrachus labrax in Israel. The disease remains asymptomatic for a long time, is virtually impossible to eradicate with antibiotics, stunts the growth of the fish and renders the fish unmarketable. The pathogen was identified as Mycobacterium marinum by direct sequencing and analysis of approximately 600 bp of the pathogen ribosomal encoding DNA (rDNA). The polymerase chain reaction technique was evaluated as a diagnostic tool for detecting the infection in D. labrax and found to be highly specific and sensitive.