Comparative proteomic analysis of tear fluid versus nasal mucus in allergic rhinitis patients and healthy controls

Abstract

Background: Allergy is a common disorder in the western world with a prevalence of 15-20%. The combination of ocular symptoms inpatients with allergic rhinitis occurs frequently. Nasal mucus for the nasal epithelium and tear fluid for the eye are the first defence barrieragainst various pathogens including aeroallergens. Little is known about the nasal mucus and particularly about the tear fluid proteome.The aim of the study was to analyse both body fluids on a proteome level and detect possible impact of its proteins in the pathophysiology in allergic rhinoconjunctivitis. Method: Fiftyeightpatients (29 allergic, 29 healthy controls) were included in this study. Allergy status wasconfirmed through symptom history and skin prick test (SPT). Patients sensitised to house dust mite or animals were excluded. Nasalmucus was collected with a special suction device, tear fluid was collected with a glass capillary. Specimens then were sent for LC MS/MS mass spectrometry. Results: In total 86 different proteins could be identified in tear fluid (267 in nasal mucus). 74 proteins could beidentified with a peptide count of >2. Considering a mean spectral count (SC) of >4 in either group 18 different proteins could beidentified. Calculating a means SC ration between allergic (A) and healthy (H) (A/H) 6 proteins were elevated in allergics (A/ H > 1) two ofwhich were significantly elevated (lactoperoxidase and prolactin inducible protein). Twelve proteins (A/H < 1) were elevated in healthycontrols four of which were significantly elevated (serum albumin, secretoglobulin familiy 1D1, prolinerichprotein 4 and mammaglobinB). With a SC of >4 and a peptide count of >1, 56 different proteins were found in tear fluid and nasal mucus, 13 of which were found in both fluids, five exclusively in tear fluid and 38 exclusively in nasal mucus. Conclusion: Tear fluid proteome is significantly different between allergics and healthy controls, significantly elevated proteins in allergics reflect exposure to exogenic noxa trough peroxidise activity and pathological condition of tissue. In healthy controls proteins reflect normal lacrimal gland secretory function and immunemodulation through steroid binding protein activity. A larger number of proteins are found in nasal mucus only (38 vs. 5), the functional differences need to be further determined.