Quantitation of hiv‐1 rna in blood cells of arc and aids patients

Abstract

Peripheral blood mononuclear cells from HIV‐1‐infected persons were mixed with 5 M guanidine thiocyanate, and HIV‐1‐specific probes were hybridized with target RNA directly in the lysate. No RNA purification was needed. Hybrids were purified by repeated capture on superparamagnetic beads coated with oligo(dT) in a device‐assisted format, a procedure termed “reversible target capture.” Blood mononuclear cells from 70 ARC and AIDS patients were examined and found to have an average of 1.8 × 105 molecules of HIV‐1 RNA per 2 × 106 cells. Copyright © 1989 Wiley Periodicals, Inc., A Wiley Company