Rapid in vitro assembly of caulobacter crescentus FtsZ protein at pH 6.5 and 7.2

Abstract

Background:Arecent study reported that Caulobacter crescentus FtsZ (CcFtsZ) required 10 min to reach a plateau, 10-100 times slower than other bacteria. Results: We assayed assembly of CcFtsZ and found that it reached steady state in 5-10 s. Conclusion: The slow kinetics in the previous study may have resulted from nucleotide deprivation. Significance: CcFtsZ assembly kinetics are similar to those of other bacteria. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.