A simple uv and derivative spectrophotometric methods for determination of antioxidant vitamin ß-carotene in spiked human plasma and vitamin drug

Abstract

A simple and sensitive UV, first- and second-order derivative spectrophotometric methods are proposed for the determination of β-carotene in spiked human plasma and vitamin drug. The quantitative determination of;β-carotene was carried out by using wavelengths 452 nm (for UV; A), 496 nm (for first-order derivative;1D) and 484 nm (for second-order derivative;2D). The methods were validated with the concentration range 0.5-5.0 ng/mL in both plasma and standard solution. The calibration curves were linear with correlation coefficients >0.999. The limits of quantitation of three methods were 0.075 μg/mL in plasma and 0.050 μg/mL in standard solution. For all methods, the recovery of β-carotene from plasma and drug mixture was higher than 80.3 % and 87.3 %, respectively. The method was successfully applied to the determination of β-carotene in pharmaceutical analysis and the results obtained from each of the three spectrophotometric methods in pharmaceutical analysis were statistically compared and no significant difference was found. © 2010, by Walter de Gruyter GmbH & Co. All rights reserved.