Mucilaginibacter gotjawali sp. Nov., isolated from soil of a lava forest

Abstract

A novel bacterial strain, designated SA3-7T, was isolated from soil of a lava forest located in Jeju, Republic of Korea. Cells of strain SA3-7T were Gram-stain-negative, oxidase-and catalasepositive, non-motile rods and produced creamy white colonies on ten-fold-diluted R2A agar. The isolate contained menaquinone-7 (MK-7) as the predominant isoprenoid quinone and summed feature 3 (C16:1ω7c/C16: 1ω6c), iso-C15: 0 and iso-C17: 0 3-OH as the major fatty acids. The DNA G+C content was 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain SA3-7T was related most closely to Mucilaginibacter frigoritolerans FT22T (96.7% sequence similarity) and that it formed a separate lineage in the genus Mucilaginibacter. Combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain SA3-7T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter gotjawali sp. nov. is proposed; the type strain is SA3-7T (=KCTC 32515T=CECT 8628T=DSM 29289T).