γδ T cells condition dentritic cells in vivo for priming pulmonary CD8 T cell responses against Mycobacterium tuberculosis

Abstract

γδ T cells and dendritic cells are quickly recruited to the lungs shortly after intranasal vaccination with BCG, but the functional in vivo interplay between these two cell populations and its role in the induction of adaptive immune responses is unclear. Using TCR-deficient mice and bone marrow chimeras, we show here that γδ T cells provide a non-redundant early source of IFN-γ in vivo, which enhances IL-12 production by lung dendritic cells. The in vivo-conditioned dendritic cells, in turn, prime a more efficient lung CD8 T cell response against Mycobacterium tuberculosis. Thus, strategies exploiting γδ T cell function and IFN-γ production could be valuable for the design and testing of mucosal vaccines. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.