Synthesis of fatty diethanolamide from Calophyllum inophyllum L. Kernel oil via enzymatic reaction

Abstract

In the present paper, fatty diethanolamide has been produced from Calophyllum inophyllum L (local name: nyamplung) kernel oil. The synthesis of fatty diethanolamide was performed by reacting triglycerides from the nyamplung kernel oil with diethanolamine catalysed by Thermomyces lanuginosus immobilized lipase (Lipozyme TL IM). The optimum reaction conditions were of the ratio of oil to diethanolamine at 1:5 (g:mmol), amount of enzyme at 0.05 g, reaction temperature at 40°C, and incubation time at 2 h. The percentage yield of fatty diethanolamide at optimum condition was 44%. Characterization of the products were performed using gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy.