Simultaneous quantitation of nicotinamide riboside and nicotinamide in dietary supplements via HPTLC-UV with confirmation by online HPTLC-ESI-MS

Abstract

The identification and quantitation of nicotinamide riboside (NAR) and its main related compound (nicotinamide) were achieved using high-performance thin-layer chromatography (HPTLC)-ultraviolet (UV) densitometry with confirmation by online electrospray ionization (ESI)-mass spectrometry (MS). As the stationary phase, HPTLC Si 60 F254 glass plates were employed; the mobile phase was ethanol-1 M ammonium acetate-formic acid (7:1:0.1, v/v/v). No derivatization was applied, and UV densitometry was performed in the absorbance mode (270 nm). The method was validated by specificity, linearity, accuracy, precision, and robustness.