Abstract
ELISPOT immunoassay, where samples are spotted onto 0.45 pm pore-size nitrocellulose and incubations are based on a BLOTTO/Tween system, proved satisfactory for detecting northern anchovy EngrauLis mordax yolk in euphausiid Euphausia pacifica guts. Polyclonal antiserum to anchovy yolk was developed in male New Zealand rabbits. The system detected 30 nannograms purified yolk protein with 1:5000 diluted antiserum and positively identified 75 '10 of experimentallyfed
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CITATION STYLE
Theilacker, G., Kimball, A., & Trimmer, J. (1986). Use of an ELISPOT immunoassay to detect euphausiid predation on larval anchovy. Marine Ecology Progress Series, 30, 127–131. https://doi.org/10.3354/meps030127
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