Tuning of Oxidation Reduction Potential of Enzyme Glucose Oxidase with Amperometry for the Estimation of Copper in Food Samples

Abstract

A new amperometric biosensor for copper determination in food samples was developed through the immobilization of glucose oxidase (GOx) in polyacrylamide gel. The response time for the reaction was studied by observing the percentage inhibition with time at 0.0125 ppm concentration of Cu2+. 2.30 minutes was the optimized response time for the estimation of Cu2+ ion in the solution. Linear range for the detection of Cu2+ by the biosensor was between 0.0125-0.1 ppm. Bioprobe was operationally stable for five days followed by a fall in activity.