In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatum

Abstract

Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1-naphthalene acetic acid) and IBA (indole-3 butyric acid) effect was studied on in vitro multiplication of shoots and rooting of Drepanostachyum falcatum. In vitro micropropagation of himalayan weeping bamboo is ex-plained by in vitro shoot induction and proliferation. Excised explant with axillary bud is surface sterilized with 0.1% HgCl2 for 10 -12 minutes, cleaned with 90% ethanol and inoculated on liquid Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP/ KIN. Effect of BAP/KIN on shoot induction is with different rate and number of shoots produced by explants with axillary bud cultured on MS media supplemented with 0.0 mg/L BAP/KIN -5.5 mg/L BAP/KIN. Shoot multiplication with highest rate is achieved on MS medium supplemented with 3.5 mg/L BAP after 4 th sub-culturing. The most effective with highest rate and number of root induc-tion combination is 6.5 mg/L IBA after 5 weeks. The roots produced by 6.5 mg/L IBA is best com-pared with other combination of auxin NAA (1-naphthalene acetic acid).