PCR Protocols—Current Methods and Applications

Abstract

The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR - PCR in detecting DNA and RNA - PCR in DNA synthesis and mutagenesis - PCR cloning of DNA - selection of primers - radioactive and nonradioactive labeling - quantitation of PCR products by HPLC - chromosome assignment - PCR mapping of HLA genes - sequencing of PCR products - genomic footprinting - quantitation of tumor gene expression - screening of phage libraries - SSP-PCR and genome walking - subcloning of PCR products.