In vitro micro-propagation of Typhonium flagelliforme (Lodd.) Blume and its genetic fidelity using ISSR and RAPD markers

Abstract

An araceous plant, Typhonium flagelliforme is in its extinct stage and require several ways to conserve its family. In the present study, an efficient In vitro protocol is established using apical meristem as an explant on basal Murashige and Skoog media supplemented with different concentrations of plant growth regulators. As an outcome, maximum number of multiple shoots (10.7±0.67) were generated on the media supplemented with 2.0 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L α-napthalene acetic acid (NAA). The regenerated shoots were further sub-cultured on the fresh media containing 0.5mg/L BAP for elongation and proliferation. Media supplemented with 1.0 mg/L Indole-3-butyric acid (IBA) and 2% sucrose enhanced rooting in the proliferated shoots. In the process, an average of 90% plantlets survived, hardened and transferred to the field conditions. Further, genetic fidelity was exhibited among the tissue culture raised plants with the markers ISSR and RAPD. Thus, the modified tissue culture protocol and its standardization would be used for large-scale propagation and conservation of this medicinal plant.