DNA isolation and amplification of Dryobalanops oblongifolia DREY and Dryobalanops lanceolata BURCK

Abstract

Dryobalanops belong to a member of Dipterocarpaceae, which has a high economic value. Due to illegal harvesting for its wood, the population tends to decrease every year, so rapid conservation effort was needed. D. oblongifolia and D. lanceolata were important members of dryobalanops, which have listed as endangered (EN), according to IUCN. This study aims to provide information on the DNA isolation and amplification process. This data was needed as basic information for further research, such as genetic diversity, population genetics, phylogeny studies, or biogeography aspects. DNA isolation was performed using the CTAB method (Cetyl Trimethyl Ammonium Bromide). The amplification process was performed with three barcode markers those were trnL-trnF, rbcL, and matK. The results of DNA isolation showed quite good DNA yield. Amplified DNA using all primers showed successfully with an annealing temperature of 490C for matK, 500C for trnL-trnF, and 550 C for rbcL.