Purification and characterization of protease from Bacillus thuringiensis isolated from soil

Abstract

Proteases are one of the most important groups of industrial enzymes. These enzymes are used in detergent, protein, meat, leather, dairy, pharmaceutical and food industry. In this study, protease enzymes produced from Bacillus thuringiensis which was isolated from soil was purified. The optimum conditions of this purified enzyme were investigated. The effect of the different production environment, different pH, different temperature degrees and different metal ions of the enzyme produced from Bacillus thuringiensis were observed. It was seen that the highest protease activity was at 55ᵒC and at pH-7.