5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs

Mark Skipsey; Gordon Hack; Thomas A. Hooper; Mark C. Shankey; Louis P. Conway; Martin Schröder; David R.W. Hodgson

Journal ArticleOPEN ACCESS

5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs

Nucleosides, Nucleotides and Nucleic Acids (2013) 32(12) 670-681

DOI: 10.1080/15257770.2013.851393

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Abstract

5'-deoxy-5'-hydrazinylguanosine was incorporated into the 5'-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5'-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay. © 2013 Copyright © Taylor and Francis Group, LLC.

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Skipsey, M., Hack, G., Hooper, T. A., Shankey, M. C., Conway, L. P., Schröder, M., & Hodgson, D. R. W. (2013). 5’-deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs. Nucleosides, Nucleotides and Nucleic Acids, 32(12), 670–681. https://doi.org/10.1080/15257770.2013.851393

5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs

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