Antioxidant activity and phytochemical analysis of Alchemilla persica Rothm

Abstract

In present study, the extracts prepared using aerial parts and roots of Alchemilla persica Rothm. were evaluated for their antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and measurement of malondialdehyde (MDA) levels. HPLC analyses of the extracts were also performed using some phenolic acid and flavonoid standards. The hydro-methanolic extract of the aerial parts was found to possess significant antioxidant activity in both assays. IC50 values for the aerial parts and roots are determined as 0.055 M and 0.151 M respectively against DPPH radical. In TBARS assay, the MDA level of the aerial parts was found to be 5.9 nmol/ml, while 19.08 nmol/ml for the root extracts.