Detection of Aflatoxin B1 through indirect ELISA from fresh grains obtained from three maize growing zones of India

Abstract

Aflatoxin B1 (AFB1) is most frequently found in plant substrates, which has shown the highest toxigenic potential. Based on previous studies, the IARC has classified AFB1 as a class 1A human carcinogen. Several impacts on consumers, such as loss of human and animal lives; health care and veterinary care costs; contaminated foods and feeds disposal costs; and investment in research and management of the myco-toxin problem. Fourteen maize seed samples comprising of recommended and local varieties were collected from three maize growing zones (Zone I- Almora, Kullu, Bilaspur, Dhaulakuan, Kangara, Saharanpur, Zone II- Karnal, Ludhiana, Pantnagar, New Delhi and Zone III- Begusarai, Varanasi, Sabour-1 and Sabour 2). In our studies AFB1 toxin range were noticed Zone-I (0.0294- 153.5081 ppb), Zone-II (0.1761- 161.0537 ppb ppb) and Zone-III (3.8366- 53.1256 ppb) collected seed samples.This indicate that ELISA technique could be applied to the monitoring of Aflatoxin contamination in a lot of samples in a cost, accuracy, simplicity and time effective manner.