Susceptibility of ferrets (Mustela putorius furo) to experimentally induced rabies with European bat lyssaviruses (EBLV)

Abstract

Twenty ferrets (Mustela putorius furo) were inoculated by intramuscular (i.m.) injection with European Bat Lyssaviruses (EBLV) type-1 and 2 using 104.0 foci-forming units (FFU) EBLV-2 (n = 6), 104.0 FFU EBLV-1 (n = 7) and 106.0 FFU EBLV-1 (n = 7). Furthermore, 15 mice received 102.5 FFU EBLV-2 (n = 5), 102.5 FFU EBLV-1 (n = 5) and 104.5 FFU EBLV-1 (n = 5) by i.m. inoculation. All ferrets and mice receiving the higher dose of EBLV-1 succumbed to infection. In contrast, only three of seven ferrets and two of five mice inoculated experimentally with the lower EBLV-1 dose died. By comparison, all of the EBLV-2 infected ferrets and four of five mice survived infection. All 20 infected ferrets seroconverted. Using sensitive molecular tools, the virus was detected in different tissues, but it could not be found in any saliva samples taken during the 84-day observation period.