Optimization of the Extraction Technology of Chlorogenic Acid in Honeysuckle Response Surface Method

Abstract

The ultrasound-assisted extraction of chlorogenic acid from honeysuckle was modeled using re-sponse surface methodology. A three-level-three-factor Box–Behnken design was employed to op-timize three extraction variables, including extraction temperature (X1), ethanol concentration (X2), and soak time (X3), for the achievement of high extraction yield of the chlorogenic acid. The optimized conditions are extract temperature of 80˚C, ethanol concentration of 70.37%, soak time of 9.35 h. Under this optimized conditions, the experimental yield of chlorogenic acid is 1.918%, which is well matched with the predicted yield of 1.929%.