Determination of LC50 value of Nicotiana tabacum L. extract against Tenebrio molitor and Zophobas morio larvae

Abstract

Tobacco has lots of active compounds that can be used as raw material for making natural insecticides. Tenebrio molitor and Zophobas morio are examples of agricultural pests. The purpose of this study was to determine LC50 values of tobacco extract on Tenebrio molitor and Zophobas morio. Previous research showed that tobacco extract had neurotoxin activity and nicotine was the highest content contained in the tobacco leaves. Tobacco extract was obtained by the Extended Heat Reflux Extraction method with ethanol solvent. LC50 values of tobacco extract for Tenebrio molitor and Zophobas morio were 21.1 mg/ml and 71.1 mg/ml, respectively.