Effect of chitosan film containing bFGF on wound healing in genetically diabetic mice

Abstract

We studied the effect of chitosan film containing bFGF on wound healing in genetically diabetic mice. Full thickness wounds of skin (2 cm2) were created on the back of each mouse. The chitosan film was prepared as follows : hydroxypropyl chitosan acetate buffer solution (6%w/v) was casted in a Teflon cast (1 × 1 cm) and dried. The wounds were treated with chitosan film, with chitosan film containing bFGF (0.6 μg/cm2and 2.0 μg/cm2), and without any film (control group). On the 7 day after wounding, wound area was reduced significantly in the groups treated with chitosan film and both of chitosan films containing bFGF compared with control group. On the 14 day after wounding, in chitosan film containing bFGF (2.0 μg/cm2) group, wound area was significantly reduced compared with the other groups. Histological examination revealed thickening of granulation tissue and an increase in the number of capillaries in chitosan film containing bFGF (2.0 2g/cm2) group. These results suggested that chitosan film containing bFGF is effective on wound healing in genetically diabetic mice in a dose-dependent fasion. © 1998, THE JAPAN SOCIETY OF DRUG DELIVERY SYSTEM. All rights reserved.