To improve pulsed-field gel electrophoresis-based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the Simpson diversity index, supported this 3-enzyme combination for improved differentiation of S. Enteritidis.
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Zheng, J., Keys, C. E., Zhao, S., Meng, J., & Brown, E. W. (2007). Enhanced subtyping scheme for Salmonella enteritidis. Emerging Infectious Diseases, 13(12), 1932–1935. https://doi.org/10.3201/eid1312.070185