ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY: A REVOLUTIONIZED LC TECHNIQUE

Abstract

High Performance Liquid Chromatography (HPLC) is a major technique for qualitative and quantitative drug analysis. More than 90% of drugs prescribed in official pharmacopoeias are being analyzed HPLC. HPLC analyzes the drug content in a sample with high degree of accuracy and precision. Due to the stringent regulatory requirements the number of samples for drug content analysis has been increased significantly. Therefore, pharmaceutical industries need a fast, accurate and affordable method for drug content analysis. Here, Ultra Performance Liquid Chromatography (U-PLC) offers an advancement of HPLC which is based on the principal of use of stationary phase consisting of particles less than 2μm. By using smaller particles; speed and peak capacity can be extended to new limits and the sample can be analyzes in a shorter period of time. It provides good resolution even for congeneric compounds. The present review discusses the various aspects of UPLC in pharmaceutical analysis.