Antidiabetic activity of green gold-silver nanocomposite with trigonella foenum graecum L. seeds extract on streptozotocin- induced diabetic rats

Abstract

The present study investigated the green synthesis of gold and silver (Au/Ag) nanocomposite using aqueous Trigonella foenum-graecum (fenugreek) seed extract and to evaluate the anti-diabetic activity of the prepared nanopcomposite. Antidiabetic potential of orally administered bulk aqueous extract of Trigonella (TBE) and Au/Ag nanocomposite with Trigonella seed extract (TN) on streptozotocin (70 mg/ kg) induced diabetic rats was assessed for a period of 7 weeks. Metformin was used as a standard drug. The results of Zetasizer and Transmission and Scanning Electron Microscopy images showed the presence of spherical to irregularly shaped green gold and silver (Au/Ag) nanopcomposite with an average size of 73.18 nm with polydispersity. A typical optical absorption peak at approximately 2.30 keV and 3.0 keV corresponded to the surface plasmon resonance property of gold and silver nanoparticles respectively. The green gold and silver (Au/Ag) nanocomposite showed a profound antihyperglycemic effect which was significantly better than all other treated groups. The results on serum ALT, creatinine levels and blood urea also showed a comparable ameliorative effect of both the bulk Trigonella seed extract (TBN) and the green nanocomposite (TN), being more efficacious than metformin. Thus, the green synthesis and application of the Au/Ag nanoparticles offer a novel approach in nanomedicine for diabetes management.